Figure 5 | Scientific Reports

Figure 5

From: A whole-cell tumor vaccine modified to express fibroblast activation protein induces antitumor immunity against both tumor cells and cancer-associated fibroblasts

Figure 5

The whole-tumor cell vaccine expressing FAP induces anti-tumor immunity via CD8+ T cell-mediated killing.

(a,b) The adoptive transfer of T cells in vivo. The suppression of s.c. tumor growth (*P < 0.05 or **P < 0.01) and a significant increase in survival (P < 0.01) were observed when the recipient mice were injected with T cells from mice immunized with the pFAP-B16F10 vaccine. (c) The abrogation of anti-tumor activity via the depletion of immune cell subsets. The depletion of CD8+ T lymphocytes resulted in the complete abrogation of the antitumor activity of the pFAP-B16F10 vaccine. (d–f) A representative in vitro CTL-mediated cytotoxicity experiment. (d) Non-transfected, (e) pVector-transfected or (f) pFAP–transfected B16F10 cells were used as target cells. T cells isolated from mice immunized with pFAP-B16F10 induced greater cytotoxicity against both FAP-positive and FAP-negative target cells than the control cells (P < 0.05) and exerted greater cytotoxicity to FAP-positive cells than to FAP-negative cells.

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