Figure 6 | Scientific Reports

Figure 6

From: A whole-cell tumor vaccine modified to express fibroblast activation protein induces antitumor immunity against both tumor cells and cancer-associated fibroblasts

Figure 6

Reduction of FAP-expressing cells and augmentation of lymphocyte infiltration. Sections of the B16F10 tumor tissues were obtained from C57BL/6J mice immunized as described.

(a) H&E staining of tumor tissues. Increased lymphocyte infiltration (black arrows) and necrotic and apoptotic tumor cells were observed in tumors from pFAP-B16F10 cell-immunized mice. Scale bar, 100 μm. (b,e) The expression of FAP in tumor tissues was quantified using Image Pro-Plus according to three parameters: integrated optical density (IOD), area and mean density = IOD sum/area. Significantly decreased expression of FAP was detected in mice vaccinated with pFAP-B16F10 cells (**P < 0.01). Scale bar, 50 μm. (c,f) Expression of collagen I in tumor tissues. Significantly decreased collagen expression (black arrows) was detected in the tumors from pFAP-B16F10 cell-immunized mice (**P < 0.01). Scale bar, 100 μm. (d,g) Staining of tumor tissues for CD8+ T cells. The number of CD8+ T cells was calculated for each group (*P < 0.05). Scale bar, 50 μm.

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