Figure 1
Functional and histological recovery of skeletal muscle after cryoinjury in irbesartan-treated mice.
(a) TA weight-to-tibia length ratios in mice treated with irbesartan or vehicle at 4 d (n = 5, in each group) and 14 d (n = 4, in each group) after cryoinjury. Data are presented as mean ± SEM. **P < 0.01. NS, not significant. (b) Total running distance during treadmill test in mice treated with irbesartan or vehicle at 14 d after cryoinjury (n = 7, in each group). Data are presented as mean ± SEM. *P < 0.05. (c) Histological sections with hematoxylin and eosin (HE) staining of TA muscles in mice treated with irbesartan or vehicle at 14 d after cryoinjury. Scale bars, 50 μm. (d) The sizes of centronuclear myofibers in TA muscles of mice treated with irbesartan or vehicle (irbesartan, n = 2,062; vehicle, n = 1,943) at 14 d after cryoinjury. Data are presented as mean ± SEM. **P < 0.01. (e) Immunostaining of TA muscles of mice treated with irbesartan or vehicle at 10 d after cryoinjury. Embryonic myosin heavy chain (eMHC) and Collagen 1 are represented in red and green, respectively. Scale bar, 50 μm. (f) Scatter plot and box plot of the sizes of eMHC + fibers in TA muscles of mice treated with irbesartan or vehicle at 10 d after cryoinjury (n = 6 sections from 3 mice in each group). **P < 0.01.
