Figure 2
From: Protein interaction patterns in different cellular environments are revealed by in-cell NMR
1H-15N SOFAST-HMQC spectra of human cells expressing double-type PFN1 mutants “AI” (a) “IP” (b) “AP” (c) and the triple-type PFN1 mutant “AIP” (d). The mutant types are color-coded as in the diagram shown in Fig. 1d. The corresponding human cell lysates are shown in Fig. S2. The crosspeaks used to estimate the fraction of visible protein over the total protein for each mutant are shown in (d), labelled according to the assignment. (e) Ratios between the signal intensity of the 1H-15N SOFAST-HMQC spectra and the normalized band intensity of a Coomassie-stained SDS-PAGE of lysates of human cells expressing each PFN1 mutant. The error bars are calculated from the uncertainty on the NMR signal intensities (calculated as standard deviation of the spectral noise).
