Figure 5

P450 genes involved in BR biosynthesis and transcriptional responses to Brz in Brachypodium and Arabidopsis.

Brachypodium BR biosynthetic genes were retrieved using the BLAST software. Similarities in protein sequences (percentage identity) are shown as a phylogenetic tree. Brachypodium genes are shown in red and Arabidopsis genes in black. log₂ ratio represents the gene expression ratio between read counts from the Brz treatment divided by the average read counts from mock 1-h and Brz+BL treatments. Stat represents the p-value of the microarray experiment, Arabidopsis det2 compared with wild-type¹¹ for Arabidopsis genes or FDR of RNA-seq data when the counts from the Brz treatment were compared with mock 1-h and Brz+BL treatments for Brachypodium genes. log₂ ratio is hatched in red if genes are up-regulated and in blue if down-regulated. cpm represents log₂ scaled read counts per million reads of RNA-seq data from mock 1-h, Brz and Brz+BL treatments.