Figure 3
B-cell phenotyping.
After 12 months of treatment, cryopreserved cells were stained to assess the evolution of different B cell subpopulations during AIT. Box plots represent the median and IQR of the percentage of cells of each phenotype. Data is shown for the CG, NRP and RP during 1-year follow-up of AIT (A) B-cells (CD19+); (B) Memory B-cells (CD27+); (C) Specific B-cells (DP+); (D) Memory Specific B-cells; (E) B-cells expressing IgE; (F) Memory B-cells expressing IgE; (G) Specific B-cells expressing IgE; (H) Memory Specific B-cells expressing IgE. Results for B cell subpopulations were expressed as percentage of CD19+ cells. Results for IgE expressing cells were expressed as percentage of each specific subpopulation. Statistical Wilcoxon and Mann-Whitney U tests were performed. The Bonferroni correction was applied for comparison of three groups and five different time points. No significant changes were observed for any group in any of the subpopulations analyzed. Only a significant higher percentage of memory B cells in RP at basal level were observed.
