Figure 4

HMGA1 overexpression impaired BAT development and function.

(A) Dysregulated biological process (BP) gene ontologies (GO) categories in BAT from aP2-HMGA1 transgenic mice (n = three mice/group). (B) Downregulated genes in BAT from transgenic mice (n = four mice/group). (C) Upregulated genes in BAT from transgenic mice (n = four mice/group). (D) Representative Westerns blots (WB) of adipogenic transcription factors in BAT and relative quantification of protein expression. Full-length blots are presented in Supplementary Figure 8. (E) Representative WB of mitochondrial OXPHOS complex proteins. Full-length blot is presented in Supplementary Figure 9. (F) Enzymatic cytochrome oxidase (COX) IV activity (n = five mice/group). (G) Enzymatic citrate synthase activity (n = five mice/group). (H) Specific triglyceride content in BAT (n = five mice/group). (I) Representative sections of BAT stained with hematoxylin/eosin (original magnification X100). Data are mean ± SEM. ^*P ≤ 0.05 and ^**P ≤ 0.01 vs. wild-type mice.