Figure 1 | Scientific Reports

Figure 1

From: Chronic TLR Stimulation Controls NLRP3 Inflammasome Activation through IL-10 Mediated Regulation of NLRP3 Expression and Caspase-8 Activation

Figure 1

Chronic LPS stimulation weakly activates NLRP3 inflammaome activation.

Wildtype (WT) BMDMs were stimulated with LPS for 4, 12 and 24 hours followed by ATP or nigericin for the last 30 minutes. (A,B) BMDMs were stimulated with LPS/ATP or LPS/Nigericin as indicated and lysates were blotted for caspase-1 and actin. Cell supernatants collected after LPS/ATP or LPS/Nigericin stimulations were analyzed by ELISA for IL-1β (C) and IL-18 (D). Solid arrow represents pro-form and open arrow represents cleaved-form of the protein in Western blots. ELISA data are presented as means ± s.e.m. of technical replicates and all data are representative of at least three independent experiments.

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