Table 2 Primers used for qPCR and cDNA amplicon lengths.

From: Titanium dioxide nanoparticles stimulate sea urchin immune cell phagocytic activity involving TLR/p38 MAPK-mediated signalling pathway

Gene

Fw 5′- 3′

Rev 5′- 3′

Amplicon size nt

Accession number

Pl-TLR

ACTGTGATTTGGAGTGGTTTAT

AGGATCAAACTCAAGAAGGGGTT

128

LK022847

Pl-NF-kB

TCCCATGGAGGACTGCCGTGTCA

TCGTTGGTTACCAAGGAGACCACA

116

HE574572

Pl-p38 MAPK

TTCACTGCCAGAGGACTTCCATCA

ATACTTGCCCATACGCTCCCGA

110

LK022846

Pl-Jun

GAGACTCAGTTCTACGAAGATTCA

AGGCAAGCTTGAGCATCTGTACGT

139

HE817756

  1. Pl-TLR region: Leucine rich repeat C-terminal domain.
  2. Pl-NF-kB region: N-terminal sub-domain of the Rel homology domain (RHD).
  3. Pl-Pl-p38 MAPK region: Catalytic domain of the Protein Kinase superfamily.
  4. Pl-Jun region: Jun-like domain.
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