Figure 1
From: A streamlined search technology for identification of synergistic drug combinations
Overview of the streamlined s-FSC technique used for in vitro drug combination optimization showing the three search steps used.
The procedure starts with the first search where the interactions of the initial set of drugs were investigated by testing 91 drug combinations for cell viability inhibition. The results are analysed using a second-order linear regression analysis that enables the elimination of the least significant compounds. In the next step (Search 2.1) the combinations of all remaining drugs or, in parallel, of the four most effective compounds (Search 2.2) identified in Search 1, were analysed. Searches 2.1 and 2.2 identified a group of four compounds, which appeared to show the strongest single drug actions, as well as synergistic interactions. A third search was therefore performed to test the interaction of these four compounds in combination. The highlighted pathway corresponds to the final s-FSC technique scheme.
