Figure 5

(a) Relative abundance of the [M-H]− ions of the different molecular species of lysophosphatidylethanolamine (LysoPE) present in embryos of Carcinus maenas and Necora puber at stage 3. (b) General structure of LysoPE. (c) Major molecular species of LysoPE identified by liquid chromatography – mass spectrometry in negative-ion mode in the embryos of C. maenas and N. puber. Error bars represent standard deviation of three independent samples. P values for each significant statistical test performed are represented in the figure with a number, with significant differences between crab species being represented on the top of the graph bars. (t-test, 1: P = 0.0435). a12,15-epoxy-13,14-dimethyl-eicosadienoate