Table 2 In vitro sensitivity of Dd2, Dd2Dd2, Dd2Dd2 L272F and GC03 to antimalarial drugs.

From: Mutations in the Plasmodium falciparum chloroquine resistance transporter, PfCRT, enlarge the parasite’s food vacuole and alter drug sensitivities

Drug†

Mean ± SEM IC50 values for individual parasite strains/lines‡

Dd2

Dd2 Dd2

Dd2 Dd2 L272F

GC03

CQ

97 ± 6.8

88 ± 6.8

20 ± 1.8*

13 ± 2.3

MDCQ

497 ± 43

440 ± 33

121 ± 9.0*

26 ± 3.0

MDAQ

45 ± 5.8

35 ± 3.6

22 ± 4.0**

18 ± 1.1

PPQ

32 ± 3.1

32 ± 5.8

39 ± 1.5

23 ± 5.3

ART

18 ± 1.1

18 ± 4.6

13 ± 1.9

15 ± 4.7

BSD

456 ± 46

631 ± 35***

708 ± 54

456 ± 59

  1. †CQ, chloroquine; MDCQ, monodesethyl chloroquine; MDAQ, monodesethyl amodiaquine; PPQ, piperaquine; ART, artemisinin; BSD, blasticidin.
  2. ‡IC50 values are listed in nM and are shown as the mean ± SEM. n = 3 independent assays (each performed in duplicate). Significantly different mean IC50 values between Dd2Dd2 and Dd2Dd2 L272F (F-test; *p < 0.0001, **p = 0.07) and between Dd2 and Dd2Dd2 (F-test; ***p = 0.038).
Back to article page