Figure 6
From: TAK1 inhibition prevents the development of autoimmune diabetes in NOD mice
TAK1 regulates Tregs generation and function.
T cells were isolated from spleens using standard procedures from 13 week-old NOD mice treated with 15 μg, or 30 μg/mouse OZ once a week from 8 until 11 weeks of age. (a) Flow cytometry of T cells population in NOD mice. Cells were stained with CD4, CD25 and Foxp3. (b,c) The proportion of T cells populations in NOD mice. (d) T cells suspensions were prepared and cultured for 3 days in 96-well culture plates coated with 3 μg/mL anti-CD3/CD28 antibodies. Supernatants were assayed for TGF-β1 production in T cells by ELISA. Data are representative of three or four independent experiments. Each symbol represents one mouse. The results of duplicate cultures are expressed as mean ± SD (n = 6 in each group). *P < 0.05, **P < 0.01 for Control vs. OZ (15 μg or 30 μg/mouse)
