Figure 5
From: Experimental Validation of Bacillus anthracis A16R Proteogenomics
Validation of expression of peptide fragment NP2.
(A) Synthetic peptide mass spectrum mapping of NP2. (Top) Spectra filtered by proteogenomic analysis. (Bottom) Spectra of synthesis peptide. (B) The encoded region of NP2. The 515-bp (top) and 1275-bp (bottom) fragments were respectively cloned and fused with the gfp gene. (C) Validation of NP2 by GFP fusion experiment. No green fluorescence was observed when the 515-bp fragment was inserted to the vector.
