Figure 6
From: Experimental Validation of Bacillus anthracis A16R Proteogenomics
Validation of peptide fragment NP3.
(A) Synthetic peptide mass spectrum mapping of NP3. (Top) Spectra filtered by proteogenomic analysis. (Bottom) Spectra of synthesis peptide. (B) The encoded region of NP3. The 615-bp length (top) and 1497-bp length (bottom) sequence were respectively cloned and fused with the gfp gene. (C) GFP signal validation of NP3. No fluorescence was observed when the 515-bp fragment was inserted into the vector.
