Figure 2 | Scientific Reports

Figure 2

From: MiR-125a-5p Decreases the Sensitivity of Treg cells Toward IL-6-Mediated Conversion by Inhibiting IL-6R and STAT3 Expression

Figure 2

miR-125a-5p is upregulated by GATA3.

(A) Luciferase assay of the promoter of miR-125a-5p in 293T cells. (B) Luciferase activity of reporter vectors with mutations in the promoter of miR-125a-5p in 293T cells. The luciferase activities were detected 48 h after transfection. (C) The expression levels of miR-125a-5p were detected by Real-Time PCR in Jurkat and Jurkat-FOXP3 cells after GATA3 overexpression. (D) After TCR activation, the Real-Time PCR measurements were measured for the expression levels of miR-125a-5p in Jurkat-FOXP3 cells and primary human nTreg cells at different time-points. (E) The expression of miR-125a-5p in GATA3-depleted Treg cells after activation. *p < 0.01 (t-test).

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