Figure 3
STAT3 and IL-6R are the targets of miR-125a-5p.
(A) The luciferase activities of wild type or site directed mutation of the 3′UTR of IL-6R in 293T cells after overexpression or knock down of miR-125a-5p, *p < 0.01(t-test). (B) The luciferase activities of wild type or site directed mutation of the 3′UTR of STAT3 in 293T cells co-transfected with ago or anta miR-125a-5p. *p < 0.01(t-test). (C) Western blot of STAT3 in primary nTreg cells with differentially expressed miR-125a-5p. Data are representative of three independent experiments with similar results. All samples of cells were derived from the same experiment and the blots were processed in parallel. Full-length blots are presented in Supplementary Fig. 2. (D) Flow cytometry assay to detect protein level of IL-6R in primary nTreg cells after overexpression or knock down of miR-125a-5p. Data are representative of three independent experiments with similar results.
