Figure 2
(a) Distribution of GC content of the 12-nucleotide random barcodes by barcode family size percentile. Data from a sequence run of healthy volunteer PBMC TCRs. (b)The correlation between the barcode family size ranking in any pair of runs for those barcodes that occur in more than one of the eight monoclonal KT2 TCR sequencing runs (Protocol A) in this study (R-squared <0.0003). Ranking is ascending and barcodes that have the same family size in a run are given the same ranking. There is no gap introduced in rankings when more than one barcode occupies a particular ranking, as such for small barcode family sizes ranking is equivalent to barcode family size. (c) For those pairs of barcodes that appear together in any pair of the eight KT2 sequencing runs (Protocol A) in this study, the relationship between the difference in barcode family sizes in one run and in the other. R-squared <0.0004. (d) Position of TCR molecules on the flowcell, coloured by barcode family size percentile. Representative example of a single frame from one flow cell from a sequencing run in this study.
