Table 1 Performance of the automated immunoassays. aThe data was collected from the Instruction for Use of respective manufactures.
Abbott | Siemens | Roche | IDS | DiaSorin | |
|---|---|---|---|---|---|
Range of detectiona (ng/mL) | 8.0–160.0 | 4.2–150.0 | 3–70 | 5.5–140 | 4–150 |
LODa (ng/mL) | 3.1 | 3.2 | 3 | — | — |
LOQa (ng/mL) | 8 | 4.2 | 5 | 5.5 | 4 |
Cross-reactivitya | |||||
25OHD3 (%) | 105 | 100.7 | 100 | 102 | 100 |
25OHD2 (%) | 82 | 104.5 | 92 | 95 | 100 |
3-epi 25OHD3 (%) | 2.7 | 1.1 | 91 | 1 | 1.3 |
Serum Pool | |||||
Pool 1 (ng/mL) | 8.6 ± 0.9 | 13.2 ± 1.7 | 6.7 ± 0.4 | 9.4 ± 1.1 | 6.5 ± 0.4 |
Pool 2 (ng/mL) | 17.6 ± 0.9 | 23.4 ± 1.4 | 16.8 ± 0.5 | 21.5 ± 1.4 | 15.6 ± 0.5 |
Pool 3 (ng/mL) | 25.8 ± 1.0 | 33.5 ± 3.2 | 26.7 ± 0.9 | 28.6 ± 0.9 | 21.6 ± 0.6 |
CV: between runs (%) | 4.4 (3.1–6.6) | 10.5 (6.4–15.9) | 3.6 (2.6–5.4) | 5.9 (3.0–9.3) | 3.3 (2.1–5.7) |
CV: total (%) | 6.9 (3.6–11.8) | 9.9 (6.1–14.1) | 4.0 (2.7–6.0) | 7.1 (3.3–11.6) | 3.7 (2.6–5.3) |
