Figure 1
From: A novel siderophore system is essential for the growth of Pseudomonas aeruginosa in airway mucus
Preparation of AMS and robust ability of P. aeruginosa to grow in AMS.
(A) SDS-PAGE analysis of two independently prepared AMS samples. Ten μg of each sample was loaded into 12% SDS-PAGE and three distinct bands were identified by Mass Spectrometry. (B) Dot-blot analysis of Muc5AC protein. AMS samples with indicated protein amount were immobilized in the nitrocellulose membrane and probed with anti-Muc5AC antibody. (C) Seven different bacterial strains (SA, Staphylococcus aureus; BC, Bacillus cereus; EC, Escherichia coli; VC, Vibrio cholerae; ST, Salmonella enterica serovar Typhimurium; LM, Listeria monocytogenes; and PAO1, P. aeruginosa) were incubated with AMS prepared from primary cultures of normal human tracheal epithelial cells for 16 h. Changes in bacterial cell viability were monitored by calculating the growth index as described in the Materials and Methods. Three independent experiments were performed and the mean values ± SD (error bars) are displayed in each bar. *p < 0.05 vs. other species, as determined by ANOVA.
