Figure 4

Growth of PAO1 pyochelin and pyoverdine mutants in AMS.

(A) Bacterial strains indicated by numbers were grown in AMS for 16 h and the growth index of each strain was measured. Experimental conditions were identical to those described in Fig. 1C. Three independent experiments were performed and mean values ± SD (error bars) are displayed in each bar. *p < 0.05 vs. growth indices of PAO1, Δpvd, Δpch and ΔpvdΔpch, as determined by students’ t-test. (B) Effect of bacterial culture supernatant (CS) on ΔPA4834 mutant growth in AMS. The ΔPA4834 mutant was grown in AMS supplemented with the CS from the ΔpvdΔpch double or ΔPA4834ΔpvdΔpch triple mutant. CSs were obtained from bacterial cultures grown in LB and diluted 10-fold in AMS. Three independent experiments were performed and mean values ± SD (error bars) are displayed in each bar. *p < 0.05 vs. growth of the ΔPA4834 mutant in AMS or AMS amended with the CS from the triple mutant, as determined by students’ t-test.