Figure 2
DWB presents predictivity to clinically-used analgesics in AIA model.
Immunized mice were challenge i.a. with 100 μg per joint of mBSA or vehicle (veh—sterile saline). Animals were pre treated with (A) indomethacin (indo—0.6, 1.8 and 5 mg/kg s.c. 30 minutes before challenge), (B) etoricoxib (etori—5, 15 or 45 mg/kg s.c. 30 minutes before challenge), (C) dexamethasone (dexa—0.5, 1 or 2 mg/kg s.c. 60 minutes before challenge), (D) methotrexate (MTX—0.6, 2 or 6 mg/kg v.o. 5 consecutive days before challenge) or (E) infliximab (inflix—5, 10 or 20 mg/kg i.p. 48 hours and 60 minutes before challenge). (F) Animals were post treated (arrow) with indo (5 mg/kg s.c.), etori (45 mg/kg s.c.), dexa (2 mg/kg s.c.) or inflix (20 mg/kg i.p.) 6 hours after mBSA challenge and the weight distribution was evaluated 2, 6 and 24 hours after drugs administration. (G) Mice were post-treated (arrow) with morphine (morph—10 mg/kg s.c.) 6 hours after mBSA challenge and weight distribution was evaluated 30, 60 and 90 minutes after drug administration. Data are means ± S.E.M. (n = 5). #P < 0.05 compared with vehicle group and *P < 0.05 compared with vehicle/mBSA group. BL (baseline).
