Figure 5 | Scientific Reports

Figure 5

From: Dynamic weight bearing is an efficient and predictable method for evaluation of arthritic nociception and its pathophysiological mechanisms in mice

Figure 5

Pharmacological inhibition of cytokines reduces joint nociception in AIA model.

Immunized mice were challenge i.a. with 100 μg per joint of mBSA or vehicle (veh—sterile saline). (A,B) Spinal cord samples were collected 3, 6, 9 and 24 hours after challenge, processed and analyzed by qRT-PCR. (A) mRNA of IL-1β and (B) TNF-α were normalized by GAPDH mRNA expression. Animals were post treated with IL-1Ra (300 ng/i.t.) or infliximab (100 μg/i.t.) 6 (C) or 24 (D) hours after challenge. Weight distribution was evaluated after 2, 6 and 24 hours after drugs administration. (A,B) Data are means ± SEM (n = 7) representative of two independent experiments. *P < 0.05 compared with vehicle. (C,D) Data are means ± SEM (n = 5). #P < 0.05 compared with vehicle group and *P < 0.05 compared with vehicle/mBSA. BL (baseline).

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