Figure 2

In vivo dynamics of transplanted Lin⁻ cells in dermatitis mice.

(a) Schematic overview of DNCB-treatment and Lin⁻ or Lin⁺ cell transplantation. Lin⁻ and Lin⁺ cells were isolated from Luc-Tg mice. (b) Bioluminescence images were taken 6 and 12 h post-transplantation of Luc-Tg cells (5 × 10⁶). Dorsal images are shown. Total photon counts were generated using the entire dorsal area of the mouse as the region of interest (ROI). (c,d) Longitudinal dorsal bioluminescence images. (c) Representative dorsal images of dermatitis or control mice transplanted with Luc-Tg Lin⁻ or Lin⁺ cells (5 × 10⁵) were taken on days 1, 3, 7, 14, 21 and 28 post-transplantation. The inflamed right ear was designated as the ROI. (d) Photon flux values emanating from the right ear of the mice were plotted longitudinally. Peak signal values were compared between experimental groups on day 14 post-transplantation. (e,f) Longitudinal ventral bioluminescence images. Ventral images were processed as above (c,d) after designation of the upper tibia as the ROI. Data shown (b–f) are representative of more than three independent experiments (n = 4 mice/group/experiment). Bar graphs in (b,d,f) are presented as means ±SEM. P values were determined using two-tailed unpaired Student’s t-tests; ns, not significant, *P < 0.05, **P < 0.01.