Figure 3

Early homing and expansion of Lin⁻ cells at the site of inflammation and BM.

(a–c) Flow cytometric analysis of CMFDA⁺ cells in inflamed skin and BM at 6 and 12 h after transplantation of CMFDA⁺CD45.1⁺Lin⁻ cells. Dermatitis or vehicle-control CD45.2⁺ B6 mice were transplanted with CMFDA-labeled Lin⁻ cells or Lin⁺ cells (1 × 10⁶) and analyzed for the presence of CMFDA⁺ cells in the inflamed skin and BM at 6 and 12 h after the transplantation by flow cytometry; representative flow cytometric data are shown. The percentage of CMFDA⁺ cells in the leukocytes present in skin (a) or BM cells (b) and the numbers of CMFDA⁺ cells in the skin and BM per mouse are shown. (c) Flow cytometric analysis of lineage marker expression in CMFDA⁺ cells in inflamed skin and BM at 6 h post-transplantation of CMFDA-labelled CD45.1⁺Lin⁻ or Lin⁺ cells. Representative data are shown. (d,e) Flow cytometric analysis of CD45.1⁺ cells in inflamed skin (d) and BM (e) 14 days post-transplantation of CD45.1⁺Lin⁻ or Lin⁺ cells; representative data are shown. Data were processed as described in (a,b). Data shown (a-e) are representative of more than three independent experiments (n = 3 mice/group/experiment). Data (a,b and d,e) are presented as means ±SEM. P values were determined using two-tailed unpaired Student’s t-tests; ns, not significant, *P < 0.05, **P < 0.01.