Figure 1 | Scientific Reports

Figure 1

From: Germ plasm localisation of the HELICc of Vasa in Drosophila: analysis of domain sufficiency and amino acids critical for localisation

Figure 1

ApVas1 was not colocalised with Oskar (Osk) to the posterior germ plasm of Drosophila oocyte.

(A) Schematic alignment of Drosophila Vasa (DmVas) and the pea aphid Vasa (ApVas1). Open boxes show the green fluorescent protein (GFP) tag, the DEAD-like helicases superfamily (DEXDc) and helicase superfamily C-terminal (HELICc) domains. Sequence identity and similarity are highlighted beneath the domain boxes. Location of the Osk interacting motif (OIM) of DmVas: amino acids 163–319. (B–D) Posterior localisation of GFP-DmVas in the oocyte of Stage-9–10 egg chambers. (E,F) Expression of GFP-ApVas1 in the late Stage-9 and Stage-10 egg chambers. Posterior localisation of GFP-ApVas1 was not identified. (B’–F’) Magnification of the insets shown in (B–F). (B”–F”) Posterior localisation of Osk. (B”’–F”’) Merged images. The egg chambers were double stained using antibodies against GFP (green) and Osk (red). In all panels, anterior is to the left and posterior is to the right. Scale bars, 25 μm.

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