Figure 2
Localisation of chimeric proteins composed of Drosophila Vasa (DmVas) and pea aphid Vasa (ApVas1) in the oocyte.
(A) Schematic illustration of domain swapping between DmVas and ApVas1. Blue and pink colours represent sequences derived from DmVas and ApVas1, respectively. The same colour codes are used in the other Fig. of this paper. (B–H’”) Localisation analysis of the green fluorescent protein (GFP)-tagged chimeric proteins in the oocytes during oogenesis from stages 9–13. (B–B”’) Posterior localisation of GFP-DmVas: a positive control. (C–C”’) GFP-ApVas1: posterior localisation was not detected. (D–D”’) GFP-DAp1 (DmVas1–157 + ApVas161–579; Osk interacting motif (OIM) was not included): posterior localisation was not detected. (E–E”’) GFP-DAp2 (DmVas1–320 + ApVas1241–579; OIM was included): posterior localisation was not detected. (F–F”’) GFP-ApD1 (ApVas11–60 + DmVas158–661; OIM was included): posterior localisation was detected. (G–G”’) GFP-ApD2 (ApVas11–135 + DmVas220–661; OIM was partially truncated): posterior localisation was detected. (H–H”’) GFP-ApD3 (ApVas11–240 + DmVas321–661; OIM was not included): posterior localisation became prominent from Stage 10a onwards. In all panels, anterior is to the left and posterior is to the right. Scale bars, 25 μm.
