Figure 3

Localisation analysis of truncated Drosophila Vasa (DmVas) proteins, chimeric Vas proteins and helicase superfamily C-terminal domains (HELICc) from other animal species.

All Vas proteins were green fluorescent protein (GFP)-tagged and analyses were performed in egg chambers from Stages 9–13 of oogenesis. Each panel shows the posterior half of the oocyte. (A–A”’) GFP-DmVas^460–661: sequence N-terminal to HELICc was truncated; (B–B”’) GFP-DmVas^{460–621/HELICc} (abbreviated as DmVas^460–621): a sole HELICc. Posterior localisation of DmVas^460–661 and DmVas^{460–621/HELICc} was detected; (C–C”’) GFP-DmVas^1–460: a C-terminal truncated DmVas polypeptide without the HELICc and C-terminal sequences. (D–D”’) GFP-DmVas^470–661: an N-terminal truncated DmVas polypeptide without the N-terminal sequence, DEXDc and 10 residues in the N-terminus of HELICc. Posterior localisation of DmVas^1–460 and DmVas^470–661 was not detected; (E–E”’) GFP-ApD^HELICc: a ApVas1–DmVas chimeric protein in which the N-terminal sequence and most of the DEXDc domain sequences of DmVas were replaced by those from ApVas1. Posterior localisation was detected; (F–F”’ to I–I”’) GFP-tagged HELICc of Vas orthologs from the pea aphid (F–F”’, GFP-ApVas1^HELICc), cricket (G–G”’, GFP-GbVas^HELICc), grasshopper (H–H”’, GFP-SgVas^HELICc) and mouse (I–I”’, GFP-Mvh^HELICc). Posterior localisation could be detected only in the egg chamber expressing SgVas^HELICc (H–H”’). In all panels, anterior is to the left and posterior is to the right. Scale bars, 25 μm.