Figure 4
Localisation analysis of truncated Drosophila Vasa (DmVas) proteins and the helicase superfamily C-terminal domain (HELICc) of grasshopper Vasa (SgVas) in the nuage.
Stage-5–6 egg chambers expressing green fluorescent protein (GFP)-tagged Vas proteins were double stained using the anti-GFP (green) and anti-Krimp antibodies (red). (A–E) GFP staining (green); (A’–E’) Krimp staining (red); (A”–E”) Merged images. For protein features, see Fig. 3. (A–A”’) Colocalisation of GFP-DmVas and Krimp to nuages surrounding the nuclear envelope of nurse cells: a positive control. (B–B”’,C–C”’) GFP-DmVas460–661 and GFP-DmVas460–621: both of these two DmVas truncations contain an intact HELICc sequence. Colocalisation was identified. (D–D”’) GFP-DmVas470–661, a truncated HELICc sequence of DmVas with a 10-amino acid deletion in its N-terminus, was not colocalised with Krimp. (E–E”’) GFP-SgVasHELICc: HELICc of grasshopper SgVas. Colocalisation with Krimp was identified. In all panels, anterior is to the left and posterior is to the right. Scale bars, 20 μm.
