Figure 7
Rescue vas mutant defects by Drosophila Vasa (DmVas) variants.
Examination of abdominal segments and pole cells in embryos of wild-type or vas mutants expressing truncated or chimeric DmVas proteins. Genotypes: (A–B”) Wild-type: Oregon-R; (C–D”) vas mutant: vasPD/vasPH165; (E–F”) vas mutants expressing GFP-DmVas, (G–H”) GFP-DmVas158–661, (I–J”) GFP-DmVas220–661, (K–L”) GFP-ApD1 (ApVas11–60 + DmVas158–661), (M–N”) GFP-Ap90D (ApVas11–90 + DmVas158–661) and (O–P”) GFP-ApD2 (ApVas11–135 + DmVas220–661). (A,C,E,G,I,K,M,O) Cuticle preparations. Anterior is at the top. (A) Wild-type. (C) vasPD/vasPH165 embryo: no abdomen. (E,G,K,M) GFP-DmVas, GFP-DmVas158–661, GFP-ApD1 and GFP-Ap90D rescued abdominal defect. (I,O) GFP-DmVas220–661 and GFP-ApD2 did not rescue abdomen formation. (B–B”,D–D”,F–F”,H–H”,J–J”,L–L”,N–N”,P–P’) Z-stacks of confocal microscopic images of cellular blastoderm embryos double stained with anti-GFP and anti-Vas antibodies to visualise GFP-Vas variants (green) and endogenous Vas (red), respectively. Posterior is to the right. (B–B”) Wild-type. (D–D”) vasPD/vasPH165 embryo: no pole cell. (F–F”) GFP-DmVas rescued pole cell formation. (H–H”,L–L”) GFP-DmVas158–661 and GFP-ApD1 partially restored the pole cell number. (J–J”,P–P”) GFP-DmVas220–661 and GFP-ApD2 could not rescue pole cell formation. (N–N”) GFP-Ap90D accumulated in the posterior pole, but no pole cell was identified in most embryos examined.
