Figure 2
From: The recombinant expression and activity detection of MAF-1 fusion protein
Identification of recombinant MAF-1 fusion protein.
(a) SDS-PAGE analysis of MAF-1 fusion protein. (Lane 1, Protein Marker. Lane 2, pET-28a(+) trantformants without IPTG induction; Lane 3, pET-28a(+) trantformants with IPTG induction. Lane 4, Supernatant of pET-28a(+)-MAF-1 induced by IPTG. Lane 5, Precipitation of pET28a(+)-MAF-1 induced by IPTG. Lane 6, pET-28a(+)-MAF-1 trantformants without IPTG induction. Lane 7, pET-28a(+)-MAF-1 trantformants with IPTG induction). (b) SDS-PAGE analysis of the purified MAF-1 fusion protein. (Lane 1, Protein Marker. Lane 2, Purified fusion protein of pET28a(+)-MAF-1). (c) RP-HPLC peaks of MAF-1 fusion protein. (d) Western Blotting result of MAF-1 fusion protein. (Lane 1, Protein Marker. Lane 2, Fusion protein of pET28a(+)-MAF-1. Lane 3, Negative control).
