Figure 7

Effect of the CCL17 neutraligand (GPN279) and of the CCL22 neutraligand (GPN136) on the inflammatory cell infiltration in the bronchoalveolar lavage (BAL) in a mouse model of allergic eosinophilic airway inflammation.

Balb/c mice were sensitized (i.p.) to OVA and challenged (i.n.) with OVA or saline in mice treated with solvent (black), GPN136 (white) or GPN279 (grey) administrated intraperitoneally (350 μmol/kg). The differential number of cells recovered in BAL fluid 24 h after the last challenge was counted by flow cytometry. Live leukocytes were identified as CD45⁺DAPI⁻ cells then differentiated into T cells (CD11b^lowCD19⁻CD3⁺), CD4⁺T cells (CD11b^lowCD19⁻CD3⁺CD4⁺), eosinophils (CD11b⁺CD11c⁻GR1⁻), neutrophils (CD11b⁺CD11c⁻GR1⁺), macrophages (CD11b⁺GR1⁻CD11c⁺F4/80⁺), dendritic cells (CD11b⁺GR1⁻CD11c⁺F4/80⁻).