Figure 2
From: Extracellular microvesicles and invadopodia mediate non-overlapping modes of tumor cell invasion
Matrix stiffness modulates cell morphology and mode of invasion.
(A) Cells transiently transfected to express cytoplasmic mCherry were grown on thin FITC-gelatin and imaged live using confocal fluorescence microscopy. A sequence of time-lapse images taken from Supplementary movie 1 is shown. Arrowheads indicate the leading edge of the cell and arrows indicate forming proteolytic puncta. Scale bar = 20 μm. (B) Cells transiently transfected to express cytoplasmic mCherry were grown in thick FITC-gelatin and imaged as in A. Scale bar = 20 μm. (C) Cells were grown on approximately 50 μm of gelatin in concentrations from 1 to 5%. The cells are shown both live using phase contrast microscopy (top) and fixed at higher magnification after staining for β1 integrin (green, bottom). Arrows indicate shed microvesicles.
