Table 2 Database Search Results of Three Technical Replicate RPLC-MSMS Datasets (D1, D2 and D3) of the E. coli Intact Proteome with a Spectrum-Level FDR of 1%. PrSMs = Protein Spectrum Matches.
From: Accurate and Efficient Resolution of Overlapping Isotopic Envelopes in Protein Tandem Mass Spectra
PrSMs | Proteo- | Sequence | Peptide | Interpreted | Interpreted | |
|---|---|---|---|---|---|---|
forms | coverage | bond | isotopic peaks | abundance | ||
(%) | coverage (%) | (%) | (%) | |||
D1 | 4478 | 103 | 76.7 | 26.0 | 61.1 | 71.5 |
D2 | 5445 | 107 | 73.4 | 26.5 | 62.9 | 73.4 |
D3 | 5392 | 105 | 73.7 | 27.4 | 61.7 | 71.5 |
5105 ± 544 | 105 ± 2 | 74.6 ± 1.8 | 26.7 ± 0.7 | 61.9 ± 0.9 | 72.1 ± 1.1 |
