Table 3 Resolving the OIPs among y10-1+, y20-2+ and y72-7+ in the HCD Spectrum of Myoglobin Using the OIE_CARE Method.*
From: Accurate and Efficient Resolution of Overlapping Isotopic Envelopes in Protein Tandem Mass Spectra
A | B | C | D | E | F | G | H | |
|---|---|---|---|---|---|---|---|---|
1 | y10-1+ | y20-2+ | y72-7+ | |||||
2 | Exp. m/z | Exp. abun. (observed) | Exp. abun. (ideal) | Exp. abun. (ideal) | Exp. abun. (ideal) | Deviation | ||
3 | 1142.617676 | 480992.312500 | 46615.469396 | 416521.895787 | 66542.651159 | −0.09 | ||
4 | y10-1+ | |||||||
5 | Theo. m/z | Theo. rel. abun. | Exp. m/z | Exp. abun. | Exp. rel. abun. | IPMD | IPAD | IPAD# |
6 | 1141.607714 | 63.64 | 1141.613770 | 128926.921875 | 63.64 | 5 | 0 | |
7 | 1142.610419 | 23.01 | 1142.617676 | 42330.617979 | 20.89 | 6 | −2 | 214 |
8 | y20-2+ | |||||||
9 | Theo. m/z | Theo. rel. abun. | Exp. m/z | Exp. abun. | Exp. rel. abun. | IPMD | IPAD | IPAD# |
10 | 1142.611961 | 100.00 | 1142.617676 | 378235.583145 | 90.81 | 5 | −9 | 15 |
11 | 1143.113357 | 67.65 | 1143.120117 | 281777.062500 | 67.65 | 6 | 0 | |
12 | y72-7+ | |||||||
13 | Theo. m/z | Theo. rel. abun. | Exp. m/z | Exp. abun. | Exp. rel. abun. | IPMD | IPAD | IPAD# |
14 | 1142.607764 | 55.06 | 1142.617676 | 60426.111376 | 50.00 | 9 | −5 | 343 |
15 | 1142.894250 | 100.00 | 1142.910522 | 120854.796875 | 100.00 | 14 | 0 | |
