Figure 1
From: PLD1 participates in BDNF-induced signalling in cortical neurons
PLD1 and RSK2 expression and PLD activity in cultured cortical neurons.
(A) E17 cortical neurons from control C57BL6 mice were cultured and lyzed between 3 and 12 DIV. 35 μg of proteins in each condition was resolved by SDS-PAGE and probed with anti-PLD1, anti-RSK2 and anti-GAPDH antibodies. The expression levels of PLD1 and RSK2 were quantified for two independent experiments and normalized to GAPDH levels. (B) WT and Rsk2−/− cortical neurons at 3 DIV were incubated for 1 to 60 minutes with 100 ng/mL of BDNF and used to measure PLD activity. Data are normalized to the activity in WT neurons in the absence of treatment and were obtained from two independent measurements with sextuplicates.
