Figure 4
From: PLD1 participates in BDNF-induced signalling in cortical neurons
PLD1 colocalizes with p-ERK1/2 and RSK2 on vesicular structures.
2 DIV WT cortical neurons were transfected with PLD1-GFP (A) or PLD1-GFP and HA-RSK2 and 24 hours later, treated for 15 minutes with 100 ng/mL BDNF. After fixation, cells were stained with anti-phospho-ERK1/2 and/or with anti-HA antibodies. Selected regions of interest revealed partial colocalization of PLD1-GFP with phospho-ERK1/2 (A) and of PLD1-GFP with HA-RSK2 and with phospho-ERK1/2 (B). Similar observations were obtained from two independent cultures. Bars = 25 μm.
