Figure 3
From: Pharmacologically targeted NMDA receptor antagonism by NitroMemantine for cerebrovascular disease
S-Nitrosylation is targeted to the NMDAR by NitroMemantine.
(a) Spontaneously hypertensive rats (SHR) were injected with a loading dose of saline, vehicle, memantine (Mem) or NitroMemantine (NitroMem) 2-h post occlusion and then sacrificed 90 min thereafter. Brain lysates were prepared and biotin switch assays performed to detect SNO-GluN1 and SNO-Drp1 from both the ipsilateral (stroke) and contralateral (non-stroke) hemispheres. (b) SNO-protein levels are presented as the ratio of ipsilateral to contralateral results above the baseline control value (mean + s.e.m.; n ≥ 4 for each value, *P < 0.03 compared to control by t- test).
