Figure 5
From: The C-terminal amyloidogenic peptide contributes to self-assembly of Avibirnavirus viral protease
Assembly greatly decreases the solubility of VP4 protein.
Plasmids pEGFP-wtVP4, pEGFP-VP4-C∆1, pEGFP-VP4-C∆1′, pEGFP-VP4-C∆2 and pEGFP-VP4-C∆3 were transfected into DF-1 cells and IBDV-infected cells were used as a control. Cells were lysed with TX-100-containing lysis buffer at 24 h after transfection or infection. The TX-100-insoluble and TX-100-soluble fractions were analyzed by 12% SDS-PAGE and Western blotting using anti-VP4 mAb. (a) Volume quantification of VP4 bands was detected using Quantity One software (N = 2). (b) Western blots of bands corresponding to VP4. Soluble VP4 protein was increased in DF-1 cells transfected with pEGFP-VP4-C∆2 and EGFP-VP4-C∆3.
