Figure 6
From: The C-terminal amyloidogenic peptide contributes to self-assembly of Avibirnavirus viral protease
Assembled VP4 structures mechanically alter cytoskeleton and nucleus.
IBDV-infected DF-1 cells (a), pEGFP-wtVP4-transfected DF-1 cells (b) and mock-infected DF-1 cells (c) were fixed for confocal analysis at 24 h and 36 h following infection or transfection. VP4 was detected with mouse anti-VP4 mAb or rabbit anti-VP4 polyclonal antibody followed by FITC-conjugated goat anti-mouse or anti-rabbit IgG. Host F-actin was probed with TRITC-phalloidin. Anti-β-tubulin mAb and TRITC-conjugated anti-mouse IgG were used to label host microtubules. Rabbit anti-vimentin mAb and TRITC-conjugated anti-rabbit IgG were used to stain intermediate filaments. Nuclear DNA was stained with DAPI. Scale bar is 7.5 μm for microtubules at 24 h and F-actin and vimentin at 36 h; scale bars are 10 μm for other panels. Ultrastructure of assembled VP4 in IBDV-infected DF-1 cells (d) and pEGFP-wtVP4-transfected DF-1 cells (e). The ultrathin section was stained with mouse anti-VP4 mAb followed by goat anti-mouse immunogold-labeled secondary antibody. Black arrowheads indicate bundles of VP4 tubules, N is nucleus and CP is cytoplasm.
