Figure 2 | Scientific Reports

Figure 2

From: Regulation of the unfolded protein response via S-nitrosylation of sensors of endoplasmic reticulum stress

Figure 2

NO modulates MPP+-induced attenuation of the IRE1α pathway.

(a) MPP+-stimulated ER stress-related gene expression. SH-SY5Y cells were incubated in MPP+ for 24 h. RT-PCR was performed using specific primers for each mRNA. (b) Cells were exposed to varying concentrations of MPP+ for 2 h and western blotting was performed using anti-phospho-IRE1α antibody. (c) Cells were preincubated with 200 μM 7-NI for 6 h and then stimulated with 500 μM MPP+ for 12 h. Splicing of XBP1 mRNA was assessed by RT-PCR. (d) Quantification of XBP1 mRNA splicing is shown in (c). Values are expressed as mean ± s.e.m. (n = 4; *P < 0.05 by ANOVA).

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