Figure 3

Prior exposure to NO inhibits ER stress-induced XBP1 mRNA splicing.
(a) NO attenuates Tg-stimulated XBP1 mRNA splicing. Cells were preincubated with 100 μM NO for 30 min and then stimulated with 10 μM Tg for 2 h. Splicing of XBP1 mRNA was assessed by performing RT-PCR. (b) Quantification of XBP1 mRNA splicing is shown for data in (a). Values are expressed as mean ± s.e.m. (n = 4; P < 0.01 by ANOVA).