Figure 5 | Scientific Reports

Figure 5

From: Regulation of the unfolded protein response via S-nitrosylation of sensors of endoplasmic reticulum stress

Figure 5

NO-induced phosphorylation of eIF2α.

(a) Effect of NO treatment on the phosphorylation of PERK and eIF2α. Cells were exposed to varying concentrations of NO for 30 min and levels of phosphorylated PERK, total PERK, phosphorylated eIF2α, total eIF2α and β-actin were detected by immunoblotting. (b) Time-dependent eIF2α phosphorylation after exposure to 100 μM NO. (c) Quantification of eIF2α phosphorylation is shown for (b). Values are mean ± s.e.m. (n = 4; P < 0.05 or 0.01 by ANOVA).

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