Figure 4
Decrease of inflammation and inflammatory mediator production by TBBt.
(A) Mice were injected with 2 mg/kg TBBt and kidney tissues were retrieved 24 h after reperfusion. Tissues were immunostained with antibodies against F4/80 or stained with naphthol AS-D chloroacetate esterase. Neutrophils are colored red. Bar = 250 μm. (B) Expression of inflammation-related genes was determined by real-time RT-PCR 1 h after reperfusion. (C) Plasma levels of TNF-α and MCP-1 were determined by ELISA 6 h after reperfusion. Values are mean ± SEM (n = 6 mice per group). *p < 0.05 and **p < 0.01 vs. sham; #p < 0.05 and ##p < 0.01 vs. VEH.
