Figure 7
Attenuation of I/R-induced oxidative stress by TBBt.
(A) Mice were injected with 2 mg/kg TBBt and kidney tissues were stained with 4-HNE 24 h after reperfusion. The area of 4-HNE positive cells was analyzed. Values are expressed as the mean ± SEM (n = 4–6 mice per group). **p < 0.01 vs. sham; #p < 0.05 vs. VEH. (B) Tissue levels of GSH, SOD and catalase were analyzed using ELISA 6 h after reperfusion. Values are expressed as the mean ± SEM (n = 12 mice per group). *p < 0.05 and **p < 0.01 vs. sham; #p < 0.05 and ##p < 0.01 vs. VEH. (C) Kidney tissues were retrieved 24 h after reperfusion and NQO1 and HO-1 levels were examined by Western blotting. Values are expressed as the mean ± SEM (n = 6 mice per group). *p < 0.05 vs. sham; #p < 0.05 vs. VEH.
