Figure 2
Molecular study of the presence of BCR-ABL1 and t(X;14)-derived fusion transcripts in diagnostic samples corresponding to the two malignancies.
(A) End-point qualitative PCR for the translocation product fusions using primers that map up and down stream of the breakpoints. For t(9;22) also nested PCR was performed. Sample legend: 1 – CML (diagnostic PB sample), 2 – T-NHL (diagnostic PB sample), 3 – H2O, * – 1st round PCR product diluted 1:100 before being used as template in nested PCR. The bands visible in the no-template control lanes are due to primer dimer. (B) Quantitative PCR for the fusion gene transcripts. A graph of relative expression and tables of average Cts are shown. The relative quantity was calculated considering the amount of BCR-ABL1, normalised to ubiquitin, as 100% in the diagnostic CML sample and t(X;14) translocation product 100% in the diagnostic lymphoma sample.
