Figure 3
FISH characterisation of diagnostic CML and PTCL samples using the commercial probes that recognise the BCR/ABL fusion or the TCRA/D split.
(A) Ideogram and a typical example of the pattern observed in pathological nuclei hybridised with the Vysis LSI BCR/ABL DC/DF probe showing one fusion, two green and one red signals corresponding to the loss of the reciprocal ABL locus. (B) Ideogram and a typical example of the pattern seen in lymphoma cells the bear the t(X;14) detected as split in the TCRA/D locus. A normal cell with intact 14q locus as testified by the vicinity of the red and green signals and an abnormal cell in which one of the two red/green pairs is separated due to the 14q break are shown. (C) Experiments performed combining the two probes. Big red signal corresponds to the ABL locus, the big green one to the BCR locus, whist the little red and green hybridisation spots correspond to the TCRA/D locus. Ideograms and examples of hybridisation are shown as well as an ideogram of an inexistent cell bearing both translocations.
