Figure 2
From: Hetero-modification of TRAIL trimer for improved drug delivery and in vivo antitumor activities
Characterization of PEG-TRAIL-vcMMAE conjugates.
(a) SDS-PAGE analysis of N109C and the outcome of different modification steps. N109C was conjugated with PEG or MMAE for 40 min after one hour reduction by TCEP (tris (2-carboxyethyl) phosphine). Serial number in bracket (e.g., (1)) indicates the modification order. Lane 6 represents the modification step that PEG was added 30 seconds ahead of MMAE. The black arrow and dashed arrow indicate PEG-N109C and N109C-vcMMAE monomer, respectively. (b) Comparing the secondary structure of TRAIL95-281, N109C, N109C-vcMMAE and PEG-N109C-vcMMAE via Circular dichroism (CD) spectroscopy.
