Table 3 Epothilone D conversion with selected P450s of S. cellulosum So ce56 (/: no conversion; *: combined yields of 26-OH epothilone D and epothilone B).
P450 | Product | Retention | Conversion of epothilone D [%] with | ||
|---|---|---|---|---|---|
time [min] | |||||
Adx4-108/AdR | Fdx2/FdR_B | Fdx8/FdR_B | |||
EpoK | Epothilone B | 6.3 | 6.8 | 24.7 | 74.4 |
CYP265A1 | 14 OH-epothilone D | 5.5 | 3.2 | 5.5 | 6.0 |
CYP266A1 | 14 OH-epothilone D | 5.5 | / | 6.9 | 2.6 |
CYP267B1 | 21-OH epothilone D | 5.1 | 3.5 | 3.2 | 5.5 |
14-OH epothilone D | 5.5 | 3.5 | 3.4 | 4.8 | |
26-OH epothilone D/epothilone B | 6.3 | 11.6* | 4.0* | 11.7* | |
Not further characterized product | 9.5 | 3.9 | 2.0 | 4.8 | |
7-ketone epothilone D | 10.9 | 6.6 | 9.3 | 8.7 | |
