Figure 4
Reduced invasion and migration capacities of IPF fibroblasts treated with 5′-azacytidine.
The invasion and migration capacities of primary IPF fibroblasts treated with 5′-azacytidine at 5 μM for 24 hours were assessed. (a) Migration capacity of IPF fibroblasts untreated and treated with 5′-azacytidine was assayed with a Boyden chamber (**P < 0.01, Data are mean and SEM, unpaired t test, n = 5 patients). (b) Representative images of migration from IPF fibroblasts untreated and treated with 5′-azacytidine (Scale bars, 200 μm). (c) Invasion capacity of IPF fibroblasts untreated and treated with 5′-azacytidine (*P < 0.05, Data are mean and SEM, unpaired t test, n = 5 patients). (d) Representative images of invasive IPF fibroblasts from IPF fibroblasts untreated and treated with 5′-azacytidine (Scale bars, 200 μm).
